ProductMycoplasma VET(qPCR)is used to determine sequences characteristic of mycoplasms related to the urogenital system in DNA preparations obtained from material taken from the dog.
Product characteristics
Package size:100 reactions
Reaction:triplex (FAM: Family MycoplasesMetamycoplasmataceae,HEX: internal control)
Internal control:exogenousEndogenous
Designation: qualitative/quantitative
Components of the set:
- Reaction mixture: contains DNA polymerase, probes and starters and other components of the qPCR reaction;
- Positive control;
- Negative control;
- Water PCR-grade;
- Internal control
Description of pathogens detected byMycoplasma VETqPCR
Several species of mycoplasm have been associated with dog reproductive problems so far. The exact role of some species in reproductive disorders remains unexplained because they are often isolated from both healthy and sick individuals. The most important species of mycoplasm include:
- Mycoplasmopsis arginini(Mycoplasma arginini)
- Mycoplasmopsis bovigentitalium(Mycoplasma bovigentitalium)
- Mycoplasmopsis edwardii(Mycoplasma edwardii)
- Mycoplasmis cynos(Mycoplasma cynos)
- Mycoplasmis felis(Mycoplasma felis)
- Mycoplasmis canis(Mycoplasma canis)
- Mycoplasmopsis maculosa(Mycoplasma maculosum)
- Mycoplasmis opalescens(Mycoplasma opalescens)
- Mesomycoplasma molare(Mycoplasma molare)
- Metamycoplasmagateae
- Metamycoplasma spumans(Mycoplasma spumans)
Bacteria of the genusMycoplasma(kind currently calledMycoplasmopsisclassMollicutesGovernmentMycoplasmalesfamilyMycoplasmataceae) were isolated from the reproductive tracts of both healthy and infertile dogs and bitches and are considered to be part of the normal vaginal flora.
The most common speciesMycoplasmaisMycoplasma canis(now called taxonomicallyMycoplasmis canis), identified in healthy and infertile dogs, including those with genitourinary diseases, whileM. edwardii(now called taxonomicallyMycoplasmopsis edwardii) is found most frequently in healthy dogs. Other species identified in dog reproductive tracts areM. molare(nowMesomycoplasma molare),M. spumans(nowMetamycoplasma spumans) andM. maculosum(nowMycoplasmopsis maculosa) which have a variable but generally lower incidence thanThe President.
Experimental studies have shown a potential roleThe Presidentas a pathogen and othersMycoplasmaspp. have been associated with infertility of dogs or toomac. Experimental and observational studies have allowed it to be established that mycoplasms can cause inflammation of the uterus, miscarriage and mortality of newborns in many different species, such as cats, cattle, sheep, goats and humans.