Test qPCR Escherichia coli Pathotypes – E. coli Typing (RVB)

Name: Test qPCR Escherichia coli Pathotypes – E. coli Typing (RVB)
Brand: RIVBIO
Price: Cena po zalogowaniu PLN
Availability: OutOfStock

The Availability Of : 🟦 On request 100 opak

Price: Cena po zalogowaniu

Leave your phone

ProductEscherichia colipathhotypes (qPCR)used to determine sequences characteristic of pathotypesEscherichia coli: ETEC, EPEC, EIEC, EAEC and EHEC/STEC in DNA formulations obtained from culture on a breeding ground.

Product characteristics

Package size:100 indications

Response_1:triplex (FAM: markerestA, HEX: Internal control (brander)UIDA), Texas Red: markereltB)

Response_2:triplex (FAM: markeraggR, HEX: Internal control (brander)UIDA), Texas Red: marker(ipaH)

Response_3:triplex (FAM: markereae, HEX: Internal control (brander)UIDA), Texas Red: markerbfpA)

Response_4:fourplex (FAM: markerstx1, HEX: Internal control (brander)UIDA), Texas Red: markerstx2, Cy5: markerrfbE_O157)

Internal control:~~exogenous~~Endogenous

Designation: qualitative/quantitative

Components of the set:

Reaction mixture: contains DNA polymerase, probes and starters and other components of the qPCR reaction;
Positive control;
Negative control;
PCR-grade water

Description of pathotypesEscherichia coli

Escherichia coli(colitis baton) is a relatively anaerobic bacteria belonging to the familyEnterobacteriaceae. It is a symbiote forming part of the intestinal microbiome of the large intestine of warm animals, including man. In the intestine, the colon baton plays a useful role in the distribution of food. It also contributes to the production of B and K vitamins.Escherichia coliit has been shown to be pathogenic to humans and animals, mainly causing gastrointestinal and urinary disorders.

Especially serious threats to humans and animals are strains causing diarrhoea. Six patotypes stand outE. colicausing diarrhoea (DEC – Diarroeagenic)E. coli):

ETEC
EPEC ( Enteropathogenic strain)
EIEC
EAEC/EAggEC
EHEC, VTEC
STEC (strain producing Shiga toxin)

Escherichia coliis the most frequently identified pathogenic species transmitted by food and causing diarrhea in children. Diarrhoea remains the second most frequent cause of mortality and global morbidity. The main burden of this disease occurs in children in South Asia, Southeast Asia and Africa. Also in the case of animals, about half of the deaths of young animals are assumed to be caused by colibacterosis.

Identification of pathotype diarrhoeaE. coliis complex because pathogenic variants cannot be separated from the commensalE. colionly on a microbiological basis. It requires molecular analysis.

Parameters :